FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27435341
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4919891.7932673043867031TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1047650.055.958079
AGAGCAC1049400.055.8234148
TCGGAAG1081600.054.136453
CGGAAGA1083600.053.9834484
ATCGGAA1091550.053.5609932
GATCGGA1110600.052.2749561
AAGAGCA1161000.050.4819877
GAAGAGC1237250.047.4829376
TCGTATG622550.045.6039844-45
CGTATGC631850.045.30171246-47
TATGCCG630200.045.13352248-49
CTCGTAT603400.044.78846744-45
TCTCGTA593700.043.64060242-43
ATCTCGT595600.042.9072842-43
GTCACGA677650.042.77078228-29
AGTCACG692450.042.01096728-29
ACGAGAT689000.041.92723532-33
CGAGATT686100.041.9036332-33
ATGCCGT677400.041.83447348-49
TCACGAG698050.041.5900430-31
Produced by FastQC (version 0.11.5)