FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29682268
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5739521.933652778824044TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1197400.056.788549
AGAGCAC1207050.056.3461468
CGGAAGA1252950.054.2227134
TCGGAAG1256800.054.0871123
ATCGGAA1269050.053.5212822
GATCGGA1291100.052.1261861
AAGAGCA1327500.051.3480767
GAAGAGC1414650.048.279986
TCGTATG722150.045.6288144-45
CGTATGC729200.045.43891546-47
TATGCCG731250.045.1221348-49
CTCGTAT696000.045.01285644-45
TCTCGTA682550.043.88844742-43
ATCTCGT689300.043.01078442-43
GTCACGA787750.042.699428-29
GGAAGAG1615350.042.4519165
CGAGATT788250.042.27143532-33
ATGCCGT779700.042.14468448-49
AGTCACG801400.042.10547328-29
TCACGAG802800.042.02226330-31
Produced by FastQC (version 0.11.5)