FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28737010
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6166392.1458008331416525TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1236550.059.9784938
GAGCACA1241550.059.7223749
TCGGAAG1282550.057.861733
CGGAAGA1285650.057.711034
ATCGGAA1291750.057.310432
GATCGGA1309350.056.2287181
AAGAGCA1357600.054.735477
GAAGAGC1429050.052.0794456
GGAAGAG1624400.045.9128535
TCGTATG785850.045.68906844-45
CGTATGC794000.045.55727446-47
TATGCCG791300.045.4448248-49
CTCGTAT753600.045.274744-45
TCTCGTA738300.044.19682742-43
ATCTCGT741950.043.5216842-43
GTCACGA854600.042.99147828-29
ATGCCGT837200.042.7519248-49
AGTCACG866300.042.63290828-29
GCCGTCT835600.042.47006250-51
CGAGATT859100.042.4136332-33
Produced by FastQC (version 0.11.5)