FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15313973
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29300.030.84825948
CGTATGC29650.030.60036746
GTATGCC36750.024.87612347
ATGCCGT38700.023.53353149
TCGTATG41250.022.16243645
CTCGTAT44000.020.85568844
GCACACG44900.020.74457411
CACACGT47150.019.60863112
ACGTCTG47900.019.22977315
TATCTCG48050.019.09738541
ACACGTC52450.017.56148713
CGGGCGC11250.017.47916815
TGCCGTC53450.017.36201550
TCTCGTA54950.016.88807543
GCCGTCT58150.015.89955151
CACGTCT60600.015.31357614
AGCACAC65250.014.85642310
GTCACAT62000.014.7458929
GGCGGGC14250.014.04134513
CCTTTAT65650.013.9776937
Produced by FastQC (version 0.11.2)