FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007592
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22500.023.76565746
TATGCCG22250.023.5673648
GTATGCC27750.019.26930447
TCGTATG29600.017.94856345
ATGCCGT29800.017.82796949
CTCGTAT36050.015.02432944
GCACACG36600.014.70384111
TATCTCG36500.014.64989641
ACGTCTG38050.014.14383915
TGCCGTC40000.013.62671250
CACACGT39750.013.6254212
CGGGCGC8950.012.72003415
GTCGGCA29800.012.6248541
ACACGTC42800.012.49329313
ACAGCGC35300.012.31387833
GCCGTCT46350.012.13203251
CCGACTG37250.012.0407762
GACTGTC42850.011.9966824
CACACTG45100.011.85643631
GCGCGCC14800.011.65513519
Produced by FastQC (version 0.11.2)