FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12124825
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23950.030.82570848
CGTATGC26250.028.51917846
GTATGCC30200.024.67468547
ATGCCGT30100.024.52743149
GCACACG37100.021.94473611
TCGTATG34650.021.60561645
CACACGT39900.020.31837712
AGTCACG38400.019.76529928
ACGAGTG38350.019.70119332
TATCTCG38450.019.38021741
ACGTCTG41850.019.20685615
CGAGTGG40300.018.74775333
CTCGTAT39750.018.48641444
TGCCGTC40650.018.41621650
ACACGTC44600.018.25455313
GTCACGA41450.018.22783729
CACGAGT44450.016.84231231
CACGTCT49850.016.40139614
GCCGTCT49600.015.51076951
TCTCGTA49300.015.25520143
Produced by FastQC (version 0.11.2)