FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12117704
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA20250.024.53151335
TATGCCG20400.024.52084548
CGTATGC21000.024.14870546
CGTACGT21750.023.315734
CCGTACG24100.021.32925833
GTATGCC24150.020.7134347
TCGTATG28000.018.48122845
ATGCCGT27350.018.16363349
TACGTAA27750.017.6526736
CGGGCCC2208.5614374E-817.2551981
ACGTAAT32700.016.03563937
GCACACG31850.016.03053911
CTCGTAT33950.014.93740544
TGCCGTC36050.014.73701350
CACACGT34900.014.62964712
ACGTCTG36000.014.18280715
ACCGTAC38000.013.98129132
CGGGCGC7250.013.32364415
GCCGTCT41350.013.01512851
ACACGTC38900.012.94799813
Produced by FastQC (version 0.11.2)