FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12572744
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19050.024.26673946
TATGCCG20350.021.86863548
GTATGCC26350.017.674647
ATGCCGT27450.016.58923149
TCGTATG28700.015.86701945
AGTCACG31700.015.23604128
GTCACGT32400.014.90686629
GCACACG32600.014.70935411
ACGTCTG35350.013.85799915
CTCGTAT33300.013.77877844
CACACGT35000.013.60214512
CGTTTCG34600.013.36073433
CGGGCGC9050.013.34200415
GTCGGCA34050.013.2773981
ACAGCGC40100.012.38852833
TGCCGTC37900.012.379251550
CGCGTAT9050.012.202821
ACACGTC39250.012.12925413
AGCGCCG41050.012.10177935
CGCGGCT30650.011.9353251
Produced by FastQC (version 0.11.2)