FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687926
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19300.020.73493646
CGGGCGC11800.020.17269515
TATGCCG19550.019.5873148
GGCGGGC13950.017.55789413
GCGGGCG15700.014.94161614
GTATGCC25900.014.91827347
TAGCCCG15800.014.84763534
ATGCCGT26950.014.20892149
TCGGGCG18250.014.17737
GGCCTTA28800.013.775228536
CCGACTG36550.013.5922342
GACTGTC41150.013.58169654
TCGTATG29500.013.44862445
ATCGGGC20400.013.3602426
GCACACG29750.013.33529511
CGGCAAT31850.013.2200731
GGCGCAT18700.013.09820817
AGTCACG32050.012.59418628
CTCGTAT31500.012.37570444
CACACGT30950.012.37230612
Produced by FastQC (version 0.11.2)