FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15060746
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22150.020.0912648
CGTATGC22250.020.00109746
GTATGCC27950.016.0455947
ATGCCGT30550.014.6798649
TCGTATG32900.013.421720545
CGGGCCC2952.3685589E-612.8686231
AGTCACG37850.011.57594528
GCACACG39850.011.51365311
CTCGTAT38850.011.45493944
TGCCGTC41300.011.44357650
GCCGGGC7250.010.9484151
CGGGAAT41650.010.8546971
ACGTCTG43750.010.72406215
TCACGTG43100.010.646090530
GTCACGT43700.010.49988429
CACACGT43700.010.49922212
CGCGGGG7301.8189894E-1210.4006681
CACGTGA44300.010.12394131
CGGGCAT23900.010.1079151
CGGGGAT23750.010.0264431
Produced by FastQC (version 0.11.2)