FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20325796
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC25350.029.80395533
ACGTCCG33650.023.1704432
TATGCCG34250.022.15923948
TCCGCAC34550.021.86708835
CGTATGC35300.021.50016646
CCGCACA35300.021.5000636
GTCCGCA37800.020.0785434
CGCACAT38650.019.63658337
GTATGCC42200.018.63858847
ATGCCGT46450.016.78473349
GCACACG50800.015.95879611
CGCGGGG7300.015.6007781
TCGTATG51300.015.33255545
ACGTCTG52750.015.04192615
CACACGT54400.014.58558412
AGTCACG55950.014.49041528
GTCACGT57300.013.72755829
CTCGTAT58450.013.22088544
TCACGTC64000.013.15297430
TGCCGTC61650.012.98208550
Produced by FastQC (version 0.11.2)