FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886993
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC26900.033.98597333
CGTATGC29550.031.40483544
TATGCCG29850.031.2047846
GTATGCC33750.027.49667545
TCGTATG39950.023.57485643
ATGCCGT39500.023.4937647
ACGTCTG43150.022.94554915
GCACACG42500.022.80967711
CCGTCCA42900.022.11435334
CACACGT46950.020.57391212
ACCCGTC45950.020.27149632
ACACGTC48400.020.10009813
CTCGTAT45550.019.54051242
TGCCGTC49200.018.72168548
CACCCGT51100.017.89085431
GCCGTCT53050.017.68814749
CACGTCT57150.017.0830214
TCACCCG55200.016.87466630
CATCTCG55450.016.36279139
CGTCCAT56650.016.32045435
Produced by FastQC (version 0.11.2)