FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12543859
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22050.032.22920648
CGTATGC22900.030.88240646
ATGCCGT30500.023.5264449
GTATGCC30250.023.37855747
TCGTATG32550.021.83277745
GCACACG35100.020.83642411
CACACGT36100.020.06787312
ACGTCTG38500.019.17576615
CTCGTAT36250.019.12849444
ACACGTC43050.017.06851413
TGCCGTC43350.016.87094950
GCCGTCT45200.016.18049851
TCTCGTA44750.015.880643
CACGTCT47150.015.58429514
GACTGTC45600.014.6772084
CCGACCG31900.014.49128243
AGCACAC53300.014.43351610
CCGACTG41050.013.951212
GTCACAT52800.013.85220529
CGAAGGG33900.013.4327648
Produced by FastQC (version 0.11.2)