FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10590810
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA110880.10469454177725783TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23900.035.3646848
CGTATGC23950.034.71467246
GTATGCC27150.031.38576747
ATGCCGT28900.029.6044849
TCGTATG30600.027.6214345
CGTATCT33550.025.70625139
CTCGTAT35400.024.16874344
CCGTATC36000.023.76514638
TATCTCG36400.023.69420841
GCACACG37000.023.49634711
TCCGTAT37100.022.96753137
CACACGT38600.022.87936212
GTTCCGT37350.022.72143635
TTCCGTA37700.022.60189436
TGCCGTC38850.022.2887850
ACGTCTG40450.021.32145715
ACACGTC42950.020.80309313
AGTTCCG41600.020.6492134
GCCGTCT42550.020.51307151
TCTCGTA44100.019.47889543
Produced by FastQC (version 0.11.2)