FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10864325
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA169780.1562729391839806TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30450.045.2054346
TATGCCG30900.044.4346448
GTATGCC35600.038.56862347
ATGCCGT38450.035.709549
TCGTATG38950.035.51746445
GCACACG42450.033.563211
CTCGTAT42700.031.2671444
CACACGT46050.031.08905612
ACGTCTG46200.030.98911515
TGCCGTC47550.029.16584650
ACACGTC49950.029.00700213
GCCGTCT47700.028.92948351
CACGTCT52450.027.36131114
TCTCGTA52500.025.69333643
AGCACAC59400.024.39236310
CGTCTGA61250.023.5436216
CACAGTC62350.022.46459431
CAGTCAC64450.022.10712227
TCCAGTC65700.022.0014725
ACAGTCA65350.021.11637932
Produced by FastQC (version 0.11.2)