FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10623639
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20200.027.8383746
CGTATGC20900.027.40131444
GTATGCC24150.023.71376845
ATGCCGT25300.022.0902247
TCGTATG28250.020.51636743
GCACACG29900.019.15279811
CACACGT30950.018.94879212
CGGGCGC6350.017.92840615
ACGTCTG31850.017.87211415
CTCGTAT32050.017.86831342
TGCCGTC34750.016.67861648
ACACGTC36950.015.59176513
GTCACCT38850.015.5406429
GACTGTC37500.015.2710914
GCCGTCT38600.014.92562949
TACCGCG16900.014.90134058
AGCGCCG29250.014.7429935
GCGCCGG30150.014.64617136
TCTCGTA40300.014.55282741
CCGACTG31400.014.282592
Produced by FastQC (version 0.11.2)