FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10455291
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG136900.13093848846483566TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25250.039.21304344
TATGCCG26650.037.5408946
GTATGCC30700.032.58889445
ATGCCGT32900.030.72369447
TCGTATG34200.029.15276143
CTCGTAT35950.027.82947242
AGTCACG37700.026.53803428
ACGTCTG38950.026.48289315
GCACACG40000.026.04620711
CACACGT41400.025.24826612
TGCCGTC39550.025.20885348
CACGGCT42100.024.01041831
GCTACAT42150.023.73536535
GTCACGG43000.023.58810429
GGCTACA42750.023.5639734
TCTCGTA42950.022.89209241
GCCGTCT44350.022.86932649
ACACGTC45650.022.74652113
CATCTCG43300.022.62705439
CGGCTAC46450.021.76144833
Produced by FastQC (version 0.11.2)