FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15544223
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27000.030.53703946
CGTATGC28700.029.0890244
GTATGCC32700.025.42522845
ATGCCGT38350.022.12903847
TCGTATG39150.022.02945943
GCACACG44600.019.72433311
CTCGTAT43200.019.16556742
ACGTCTG45900.018.78983315
CACACGT46850.018.62960812
TATCTCG46100.018.18407439
CACTAGC48200.017.39260531
ACACGTC52000.017.11621713
TGCCGTC52550.016.74013148
TAGCTTA51350.016.39243934
CCGACTG49800.016.2806552
GCCGTCT56150.015.789680549
TCACTAG54900.015.52156830
GACTGTC58150.015.4256814
CACGTCT57800.015.39872314
CGCGCGT26100.015.0744541
Produced by FastQC (version 0.11.2)