FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18425102
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG191270.10380946602086653TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38450.037.50465844
TATGCCG41500.034.748146
ATGCCGT50800.029.13367747
GTATGCC51000.028.41085845
TCGTATG53000.027.2084943
GCACACG60050.024.76087411
AGTCACG59700.024.21312528
ACGTCTG61350.024.0115915
CTCGTAT61150.022.84875942
TGCCGTC65150.022.82263448
CACACGT65300.022.45303712
GTCACGA67350.021.36035229
GCCGTCT72150.021.03854649
ACACGTC72450.020.09432613
CACGTCT77950.019.0750314
TCTCGTA78150.018.31975441
AGCACAC86150.017.6999110
CACGATC85900.017.1090731
CGATCAG84750.017.09684833
CGTCTGA90800.016.52760116
Produced by FastQC (version 0.11.2)