FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16382925
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC35100.033.41776344
TATGCCG35200.032.5386646
GTATGCC40100.029.50905645
ATGCCGT45200.026.2556647
TCGTATG45200.026.17933543
GCACACG51450.023.1999511
CTCGTAT52150.022.29344442
ACGTCTG56050.021.97297515
CACACGT56100.021.58422912
TGCCGTC56800.020.52916348
ACACGTC59100.020.43021213
GTCACAC67700.018.09077529
CACGTCT67550.018.027714
GCCGTCT63550.018.02293449
TCTCGTA66050.017.70621941
CGTCTGA76950.016.13955316
AGCACAC76750.015.91186810
CAGTCAC84550.014.56675627
CGCGGGG6800.013.7032051
CACACTT90300.013.67748331
Produced by FastQC (version 0.11.2)