FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15409394
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG168640.10943973526798002TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31850.038.45195846
CGTATGC34550.035.5468844
GTATGCC38900.032.1926445
ATGCCGT43750.027.91399247
TCGTATG46900.026.77543
GCACACG50550.025.25100511
ACGTCTG51750.024.93204915
CACACGT54900.023.43853212
CTCGTAT54100.023.02029442
ACACGTC57400.022.65787113
TGCCGTC54700.022.64150248
GCCGTCT57100.022.23360349
TCTCGTA60400.020.79045341
CACGTCT63900.020.35314814
CATCTCG64200.019.39859639
AGCACAC72850.018.37391510
AGTCACC72350.017.50035328
CGTCTGA76250.017.23785416
CAGTCAC76700.017.22757127
TCCAGTC84150.015.62017525
Produced by FastQC (version 0.11.2)