FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15291577
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG306200.20024095618130164TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49800.044.6816146
CGTATGC51650.042.81431644
GTCACGC53450.041.696329
GTATGCC55850.040.2740945
ATGCCGT59150.037.85196347
TCGTATG60900.036.3678543
CACGCCA62100.035.66625631
TCACGCC62850.035.40532330
CTCGTAT65650.033.94637742
GCACACG67550.033.91104511
AGTCACG66200.033.82222728
ACGTCTG68600.032.9398315
CACACGT69750.032.54458612
TATCTCG69350.032.13514339
CGCCAAT69350.032.0865533
TGCCGTC71100.031.78150648
GCCAATA71900.030.9480734
ACGCCAA72700.030.84538732
GCCGTCT76350.029.64122449
ACACGTC77800.029.3543613
Produced by FastQC (version 0.11.2)