FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15267041
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31200.032.9505146
CGTATGC31800.032.1123644
GTATGCC35950.028.78922545
TCGTATG43250.024.2490743
ATGCCGT42050.024.20213347
GCACACG49300.021.41266411
CTCGTAT47100.021.24133742
ACGTCTG50850.020.82801615
CACACGT51100.020.455612
TGCCGTC53350.019.52849448
CACAGTG57300.018.30317933
ACACGTC56100.018.07896413
TCTCGTA57500.017.39914141
GCCGTCT60750.017.09281349
CACACAG63400.016.81416731
GTCACAC61950.016.5394629
CACGTCT64450.016.2185914
CGTCTGA67450.015.8555116
ACACAGT71600.014.93675632
AGCACAC71350.014.89199710
Produced by FastQC (version 0.11.2)