FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16933839
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG580960.3430763691564565TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC83800.053.88836344
TATGCCG84800.053.29341546
GTATGCC93250.048.6862645
ATGCCGT94450.047.95812247
TCGTATG96000.047.03991743
ACTGACC98800.045.77744332
CTCGTAT101500.042.85937542
TGCCGTC107700.042.15407648
ACGTCTG110550.041.41060315
CACACGT113200.040.56260712
GCACACG113650.040.43211711
CACTGAC114950.039.55633531
GCCGTCT115750.039.10307749
TCTCGTA113900.038.0721241
ACACGTC121650.037.82980713
CACGTCT125000.036.81618514
CGTCTGA133800.034.36961716
AGTCACT134400.034.0632828
GTCACTG137450.033.0562229
CAGTCAC139650.032.85671627
Produced by FastQC (version 0.11.2)