FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14799508
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG175800.11878773267327536TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34600.037.9886146
CGTATGC35800.036.61888544
GTATGCC41450.032.04355645
ATGCCGT42700.030.78192547
TCGTATG45950.028.60523243
TAGGCAT47300.027.42375235
ACTTAGG49950.025.90038132
TTAGGCA51500.025.52227434
CACTTAG51350.025.39587831
CTCGTAT53300.024.59571642
TGCCGTC54950.024.2336248
GCACACG55600.023.95031411
ACGTCTG56300.023.6532515
CACACGT59950.022.32755912
CTTAGGC59700.021.9593633
GCCGTCT60850.021.82716649
TCTCGTA62500.021.08569341
ACACGTC63750.020.94261613
CATCTCG63500.020.86194239
GGCATCT65100.019.8723437
Produced by FastQC (version 0.11.2)