FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19372243
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG284090.1466479643064564TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC52750.039.8940844
TATGCCG53250.039.6489646
GTATGCC62800.033.9492345
ATGCCGT63900.033.36446847
TCGTATG67450.031.71098143
CTCGTAT71850.030.05720942
TATCTCG71900.029.36403839
GCACACG75950.028.79786711
ACGTCTG77300.028.027315
TGCCGTC76350.027.92389948
CGATGTA79200.027.22432134
CACACGT81450.026.68390812
CCGATGT81750.026.50199533
ACACGTC87750.024.61075613
TCTCGTA90700.024.03869441
GCCGTCT90250.023.96717849
CACGTCT93100.023.49281514
AGCACAC102700.021.49845510
CGTCTGA104150.021.09983316
ACCGATG106700.020.46671932
Produced by FastQC (version 0.11.2)