FastQCFastQC Report
Thu 9 Mar 2017
HYFNLBGXY_n01_jes_021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNLBGXY_n01_jes_021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17924174
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAA221560.1236096012011488No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT218790.12206420223325214No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC212570.11859402837754197No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAAT193050.10770370785286953No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT188270.1050369182981598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28050.038.74208544
TATGCCG28900.037.36386546
GTATGCC31800.033.95646745
TCGTATG35750.030.68704643
ATGCCGT36150.029.96568347
CCTGCCA58250.028.0263671
CTGCCAG57550.027.6368622
CTCGTAT39200.027.4580842
ACGTCTG42500.026.86788215
GCACACG43550.026.06180611
CACACGT47650.023.60201512
GCCGTCT48500.022.6906549
TGCCAGT72450.022.5237833
GCCAGTA74650.021.628794
CACGTCT53350.021.20972314
TGCGCCG48550.021.10390314
GTCACAT54600.019.90354529
AGCACAC59250.019.67994510
GCGCCGA51950.019.58983615
CCAGTAG85900.018.997285
Produced by FastQC (version 0.11.2)