FastQCFastQC Report
Thu 14 Mar 2024
HYFNCDRX3_n02_CITE-seq_test_TCells_LiveFACS_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNCDRX3_n02_CITE-seq_test_TCells_LiveFACS_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681554
Sequences flagged as poor quality0
Sequence length90
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1597665.957963181050988No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1016403.7903394822554386No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC803382.9959493636898604No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC504681.8820430243060553No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT115910.4322493598860959No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC75750.28248545432984007No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT58460.21800791630524688No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG52260.1948869946307253No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG51170.1908221874331078No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG50670.18895759697548511No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACTTTTTTCAGGTC45750.17061002687247767No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA45600.17005064973519085No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA43020.16042936297385768No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC38330.1429395044813567No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC37430.13958324165763583No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC34930.1302602893695223No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG33800.12604631493529497No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT32500.12119837974547594No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC29790.11109229946516087No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA29440.10978708614482496No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA27510.10258976697840132No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA27360.10203038984111451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)