FastQCFastQC Report
Thu 14 Mar 2024
HYFNCDRX3_n02_CITE-seq_test_BCells_Mix_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNCDRX3_n02_CITE-seq_test_BCells_Mix_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605625
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT712202.7333173422883186No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA320371.2295322619333173No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226130.8678532022067642Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT166220.637927560566083No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT162200.622499400335812No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC80470.3088318541616695No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC65280.2505349004557448No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT62120.23840729191652674No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG58210.2234012952746462No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG46520.17853681938114657No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT46170.17719357159990407No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT45310.17389301990885106No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG42790.164221635883905No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATCGAAGTTATT28810.11056848165027584No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGCTAGAAGTTATT28300.10861117774046533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)