FastQCFastQC Report
Thu 14 Mar 2024
HYFNCDRX3_n01_CITE-seq_test_TCells_MixFACS_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNCDRX3_n01_CITE-seq_test_TCells_MixFACS_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368460
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAGACATGAGCGACTCAGTGAGT68681.863974379851273No Hit
TGACTAGAGGGCACTAACTCCCGCAG48301.3108614232209739No Hit
GAGGTGATCTCGTTTAAAAGGACAGC34720.9423003853878305No Hit
CCACTACTCAGCAACTCTATGCATGC34200.9281875915974597No Hit
TATGCCCTCTGCTTGCACACCTTAGG28880.7838028551267439No Hit
TGACAGAGGGCACTATTTATACTGCT28480.7729468599033816No Hit
TGCCCATGTGCAGTAGGGCGAACCGG28250.7667046626499484No Hit
GAGCAGACATGAGCGAGAACGTTGCG27660.7506920696954893No Hit
ACTGAGTTCGGCATCGCTTTCGGGTT27540.7474352711284807No Hit
TATGCCCTCTGCTTGCACCCTCCGCC27120.7360364761439505No Hit
CGTCACTTCCACGAATCTGTGATAGT27020.73332247733811No Hit
GTCACTTCCACGAATGGCCCCGCCTT26870.7292514791293492No Hit
TTTCCTCTCAGGCGAACCTTGTTTCC26430.7173098843836508No Hit
TGTACCCAAGCGAGTCAATAAAGCCT25880.7023828909515281No Hit
TTTATGCCAGCTCGCACGTGGGCTTA24840.6741573033707865No Hit
CCACTACTCAGCAACTGGATTTACAG24010.6516311132823102No Hit
TTTCCTCTCAGGCGAACTGGAACAGC23690.6429463171036205No Hit
TCTATTGAGATGTGTATTATGTCTGG23620.6410465179395322No Hit
TCTATTGAGATGTGTAAAATTCGCAC23160.6285621234326657No Hit
GAGGTGATCTCGTTTAAACTCTCAGA22890.6212343266568963No Hit
CAGTAACTCCGCAAGCATCGGCACTC22340.6063073332247734No Hit
TAGTTGGTCACGACTAACCACCGGCC21850.5930087390761548No Hit
TCTCATAGTTGGTTTGGGCTGGTCGC21530.5843239428974651No Hit
TAGTTGGTCACGACTAGCAACTGCTT21260.5769961461216957No Hit
ACTGAGTTCGGCATCGAGCTTGTAGT20930.568039950062422No Hit
TGCCCATGTGCAGTAGAAGGCATTCG20410.5539271562720512No Hit
CCACTACTCAGCAACTCAGACCTGCT20380.553112956630299No Hit
CCACTACTCAGCAACTCACACCGGGC20220.5487705585409542No Hit
GAGCAGACATGAGCGATGCCGGTCTG19750.5360147641535038No Hit
TCTATTGAGATGTGTACCGGTCTGAC19110.5186451717961245No Hit
TCTTTGAGATGTGTAATCAACCGTCT18270.495847581827064No Hit
GTCACAAAGAATTCCCCGAGTCACGC17520.4754925907832601No Hit
AGCAGCCCATCCAACATCGGTCTCTC16820.45649459914237644No Hit
CCTACCAGTGTTAAGACGAGTACTAC16370.444281604516094No Hit
GTGGGTCGTCTGGGTTCCTTATCGCT16340.4434674048743419No Hit
GCTCTACATGGATGGGCTACCCCGGC15220.41307061824892793No Hit
TGACTAGAGGGCACTAGCGGAACGTC15000.4070998208760788No Hit
CCACTACTCAGCAACTTATGCTTGTC14660.39787222493622104No Hit
CCACTACTCAGCAACTCGCAACGCAG14650.39760082505563693No Hit
TGACTAGAGGGCACTACACCAGCTCG14630.39705802529446893No Hit
GACACGCTCGTACCGGTGTCAGTCGT14520.39407262660804426No Hit
GACACGCTCGTACCGGACTTTGAGGT14510.3938012267274602No Hit
TTTATGCCAGCTCGCAATCGGAGGCC14340.3891874287575313No Hit
TAGTTGGTCACGACTATCCGGGTTCT14250.3867448298322749No Hit
TTTATGCCAGCTCGCACATGGTGTTT14190.3851164305487706No Hit
TTTATGCCAGCTCGCACTTCGGGACA14190.3851164305487706No Hit
CAGTCCTGTCAATGTCTGTTACGTCG14180.3848450306681865No Hit
GAGGTGATCTCGTTTAGGCATTAGGT14090.38240243174293004No Hit
GAACATCGTACCGGCTGCGCAAACCG14050.3813168322205938No Hit
TTTCCTCTCAGGCGAACTCCAACATT14020.38050263257884165No Hit
GCCTCTACATGGATGGGCCTCCGGCC14010.3802312326982576No Hit
GACACGCTCGTACCGGTCAATTCTGC13950.3786028334147533No Hit
TTTCCTCTCAGGCGAACATGCGCTAC13900.37724583401183304No Hit
CCACTACTCAGCAACTAGAGACACAT13850.37588883460891276No Hit
TTTCCTCTCAGGCGAATTTTATGGGA13780.3739890354448244No Hit
GACACGCTCGTACCGGTCAGAGTCCC13620.3696466373554796No Hit
GAGCAGACATGAGCGATGGCAAGTTC13590.36883243771372737No Hit
GAGGTGATCTCGTTTAGCGTTAAGCA13530.3672040384302231No Hit
TCTATTGAGATGTGTAACCCACTGCG13470.36557563914671876No Hit
TTTCCTCTCAGGCGAATATGGGCGGA13180.35770504260978125No Hit
TCTATTGAGATGTGTACCGCGGGGCT13060.35444824404277264No Hit
GCCTCTACATGGATGGCGTCCATATC13060.35444824404277264No Hit
TTTCCTCTCAGGCGAATATCGTGAGT13040.35390544428160453No Hit
TAGTTGGTCACGACTAAAGGGGCTTA12950.35146284535634803No Hit
CAAGAAATCGTTTAGGATTAGAGGTA12930.3509200455951799No Hit
CAAGAAATCGTTTAGGAGAGCCTCAG12920.35064864571459586No Hit
GAACATCGTACCGGCTTAAAATGTCC12900.3501058459534278No Hit
GACACGCTCGTACCGGTCCAGCCTGG12840.3484774466699235No Hit
CCACTACTCAGCAACTCAAATGTGCC12830.3482060467893394No Hit
TTTCCTCTCAGGCGAACCGTTATTTC12720.3452206481029148No Hit
TCTATTGAGATGTGTACCAAGCGAGT12710.3449492482223308No Hit
GAACATCGTACCGGCTCGTCAGGGGT12650.3433208489388265No Hit
GCCTCTACATGGATGGAATTAAGTCT12640.3430494490582424No Hit
ACAGCTATCATGCAACTGTCCCGGAT12540.34033545025240186No Hit
CCACTACTCAGCAACTCCCTCTGTCG12480.3387070509688976No Hit
GCCTCTACATGGATGGTTATACGGGA12470.3384356510883135No Hit
TTTATGCCAGCTCGCAACGGCCCAGA12450.3378928513271454No Hit
GATCGCGAGGCTAGGTTCCGCAGGAC12410.3368072518048092No Hit
GAGGTGATCTCGTTTAGACGACATCG12340.3349074526407208No Hit
GACGGCTTCGCCCTTAGCCCGCCACC12330.3346360527601368No Hit
GCCTCTACATGGATGGCGAGTCGCTG12320.33436465287955275No Hit
CCACTACTCAGCAACTTAGGTCGGAG12100.3283938555067036No Hit
TTTATGCCAGCTCGCACTCTGAGACG12080.32785105574553547No Hit
TTTCCTCTCAGGCGAAGGCAGGCTCT12070.3275796558649514No Hit
TTTATGCCAGCTCGCACGCGTAACGC12040.3267654562231993No Hit
CCACTACTCAGCAACTAAACACGGTC11950.32432285729794275No Hit
GAACATCGTACCGGCTGGACCCTCCT11920.32350865765619063No Hit
TTTCCTCTCAGGCGAACGCATTACGT11900.3229658578950225No Hit
TAGTTGGTCACGACTAAGGCTTTGTG11860.3218802583726863No Hit
TTTCCTCTCAGGCGAACAGATTGTGT11790.3199804592085979No Hit
TGACTAGGTAGAGCTGCCACCAACGG11780.3197090593280139No Hit
CCACTACTCAGCAACTGCTGGTGGCA11730.31835205992509363No Hit
GAACATCGTACCGGCTGAGGTCGACA11700.31753786028334147No Hit
TGACTAGAGGGCACTATAAAGGATTC11680.31699506052217336No Hit
TTTCCTCTCAGGCGAATTAGTTGAAT11550.3134668620745807No Hit
GAGCAGACATGAGCGAGGGTCCAAGA11480.3115670629104923No Hit
CCACTACTCAGCAACTCCCGTTCATC11460.31102426314932424No Hit
CCACTACTCAGCAACTGCAGATGATT11460.31102426314932424No Hit
AGCAGCCCATCCAACAAATACGTCCG11410.30966726374640396No Hit
CCACTACTCAGCAACTAGCCCCTAGA11280.30613906529881124No Hit
GAGGTGATCTCGTTTAGTACCAGTTA11280.30613906529881124No Hit
CCACTACTCAGCAACTAACAACTCGC11250.3053248656570591No Hit
CCACTACTCAGCAACTCCGGCTGTTT11210.3042392661347229No Hit
GATCGCGAGGCTAGGTGCATGGACAA11150.3026108668512186No Hit
GGACAGAAGTCGAGTGATTTTTTTCC11130.30206806709005046No Hit
TGACTAGGTAGAGCTGATGTACCACG11130.30206806709005046No Hit
GAGCAGACATGAGCGATCGCAGACTA11050.299896868045378No Hit
CAGATCACAGTATAAGGGGCCTAGTT11010.29881126852304185No Hit
GATCGCGAGGCTAGGTTCAGTCCATC10870.2950116701948651No Hit
TGACTAGGTAGAGCTGCAGATGCTAT10850.294468870433697No Hit
GATCGCGAGGCTAGGTAATTGGATCG10850.294468870433697No Hit
CAAGAAATCGTTTAGGCGGAAAGTGG10820.29365467079194485No Hit
GAGGTGATCTCGTTTACCCCGTGCTC10780.2925690712696086No Hit
TGACTAGGTAGAGCTGGCACCTGTCA10730.29121207186668835No Hit
GACGGCTTCGCCCTTATCCACCCGTC10720.29094067198610435No Hit
TTTATGCCAGCTCGCAATTTCCTCAA10680.2898550724637681No Hit
TCTATTGAGATGTGTAACACAAGACT10530.28578407425500735No Hit
TTTATGCCAGCTCGCATCTCACGGTT10530.28578407425500735No Hit
GAGCAGACATGAGCGAGGCAGCTCAC10330.2803560766433263No Hit
CCACTACTCAGCAACTTTTCCAACCG10300.2795418770015741No Hit
GAGCAGACATGAGCGATTCCACCGCC10290.27927047712099007No Hit
TGACTAGAGGGCACTACATGTAAGGC10230.27764207783748573No Hit
TGACTAGGTAGAGCTGGGTTCGTCTT10180.27628507843456546No Hit
TTTATGCCAGCTCGCACATCTTTGGG10160.2757422786733974No Hit
GACGTTATCATTGCGAGGTCCGTTTG10160.2757422786733974No Hit
TTTATGCCAGCTCGCAGACTTGGAAA10120.2746566791510612No Hit
TTTCCTCTCAGGCGAACCTACAACTG10080.27357107962872496No Hit
GAGGTGATCTCGTTTACTGCTCAAGC10050.27275687998697284No Hit
GAGGTGATCTCGTTTAAATAATCCCT10000.27139988058405257No Hit
GAGCAGACATGAGCGAGTCCCTCTTT9970.2705856809423004No Hit
TGACTAGGTAGAGCTGCTAAGCGGGG9860.2676002822558758No Hit
TGACTAGGTAGAGCTGTTATTGCAAC9820.2665146827335396No Hit
CCACTACTCAGCAACTGAGCTAAGCG9780.2654290832112034No Hit
GAGCAGACATGAGCGACGAAGGGCGG9740.2643434836888672No Hit
GCTGGGTAGCCAGAACACAAAGTCTA9730.2640720838082831No Hit
TCTATTGAGATGTGTAGCCCATTAGC9720.26380068392769906No Hit
TCTATTGAGATGTGTAATCTACTGAC9660.2621722846441948No Hit
TTTATGCCAGCTCGCATGGACTTACA9630.2613580850024426No Hit
TGACTAGGTAGAGCTGAGCAGCCTCT9510.25810128643543395No Hit
CCACTACTCAGCAACTCTTTGGTACG9500.25782988655484995No Hit
TGACTAGGTAGAGCTGGATATCCAAC9470.2570156869130978No Hit
CAGATCACAGTATAAGCCCTTTCACA9420.2556586875101775No Hit
TAGTTGGTCACGACTATCGACTTACA9380.2545730879878413No Hit
GATCGCGAGGCTAGGTTTCGTTGCGG9300.2524018889431689No Hit
TGACTAGGTAGAGCTGGGCGTGTAAG9250.25104488954024856No Hit
TTTCCTCTCAGGCGAATCCTTATTCA9070.24615969168973567No Hit
TAGTTGGTCACGACTACATCTCGCAT8970.24344569288389512No Hit
GACACGCTCGTACCGGCTTCGTGCTC8950.24290289312272703No Hit
CCACTACTCAGCAACTTTCGCTTCGT8870.2407316940780546No Hit
TGACTAGGTAGAGCTGGAGGACGCTG8820.23937469467513436No Hit
AGCAGCCCATCCAACATGGGAACGAG8770.23801769527221406No Hit
TAGTTGGTCCAGTAGTTTGTTAAGGC8750.23747489551104597No Hit
GCTCTACATGGATGGGCGCGGGCCTT8690.23584649622754167No Hit
CGTCACTTCCACGAATCGGCGTATGG8550.23204689789936492No Hit
GCCTCTACATGGATGGTTTATTGGAA8540.23177549801878086No Hit
GACGGCTTCGCCCTTAAAAAAGCGAA8490.2304184986158606No Hit
TCTATTGAGATGTGTATCAGTACCAC8490.2304184986158606No Hit
CAGATCACAGTATAAGGCATCTTATA8460.22960429897410845No Hit
TCTATTGAGATGTGTACGTAAGCAGC8430.2287900993323563No Hit
GCTCTACATGGATGGATCTCCTTGAT8340.22634750040709983No Hit
GCCTCTACATGGATGGCGCACTGGGA8300.2252619008847636No Hit
GGGCACTTCCGTACAACCTAAAAGCA8260.2241763013624274No Hit
GCCTCTACATGGATGGAACCCCGGCC8200.22254790207892308No Hit
AGCAGCCCATCCAACACCGGTCCATC8190.22227650219833903No Hit
GCCTCTACATGGATGGCTCTGACGCG8170.22173370243717094No Hit
CGTCACTTCCACGAATATCTGCGGTC8090.2195625033924985No Hit
TCTATTGAGATGTGTAGGTTCTTAGC7860.21332030613906533No Hit
CGTCACTTCCACGAATTAAGAACGCT7820.2122347066167291No Hit
GCCTCTACATGGATGGCAATTGTCCG7660.20789230852738425No Hit
TGACTAGGTAGAGCTGGTGGACGGAC7620.20680670900504802No Hit
GCCTCTACATGGATGGGCCGGTGGAG7600.20626390924387994No Hit
TAGTTGGTCACGACTAGACACTTGTC7570.2054497096021278No Hit
TCTCATAGTTGGTTTGCAGTCGAACT7510.20382131031862344No Hit
GTGGCGTCTAGGTTACCCTAATACTT7510.20382131031862344No Hit
GAGCAGACATGAGCGACCATGCTACA7480.20300711067687127No Hit
GATCGCGAGGCTAGGTAAACAGTCGG7470.20273571079628727No Hit
TGACTAGGTAGAGCTGTATCCTCTCG7450.20219291103511913No Hit
GAGCAGACATGAGCGACAATCGCCGA7400.20083591163219888No Hit
GGACAGAAGTCGAGTGGGCTGCTGCG7390.20056451175161483No Hit
GAGGTGATCTCGTTTACAGGGCCACT7250.1967649134234381No Hit
TAGTTGGTCACGACTATGACTTTCCT7160.19432231449818163No Hit
CAAGAAATCGTTTAGGCGGCATGACA7130.19350811485642946No Hit
TATGCCCTCTGCTTGCGTGGGGCCAT7110.19296531509526135No Hit
GAGGTGATCTCGTTTATATAAAACAA6960.18889431688650057No Hit
CCACTACTCAGCAACTCAGCCGTGTC6950.18862291700591652No Hit
GCCTCTACATGGATGGATCATTCCCA6950.18862291700591652No Hit
TCTATTGAGATGTGTATGGCGTATCA6730.18265211963306735No Hit
GAGGTGATCTCGTTTAGTCCTTACGC6720.1823807197524833No Hit
GATCGCGAGGCTAGGTAAGAAGCGCA6630.17993812082722685No Hit
TTTCCTCTCAGGCGAAGGTGCAATTT6600.17912392118547468No Hit
CGAACATTCTCTGTCGACGTTCGCGC6560.17803832166313846No Hit
TGACTAGAGGGCACTAGATCGGTCAT6540.17749552190197035No Hit
GATCGCGAGGCTAGGTAACGAACAAG6390.17342452369320957No Hit
GATCGCGAGGCTAGGTACCGCTAACG6270.17016772512620093No Hit
GACACGCTCGTACCGGAGTGCGCTAA6250.16962492536503282No Hit
CGTCACTTCCACGAATGTAGACTAGT6240.1693535254844488No Hit
GAGGTGATCTCGTTTAATTTGAGGGT6220.16881072572328068No Hit
CAGATCACAGTATAAGCTGTCACTGT6200.16826792596211257No Hit
CCACTACTCAGCAACTCTTGGTTCAG6120.16609672691744015No Hit
CGAACATTCTCTGTCGACCTGGATTA6060.16446832763393585No Hit
TTTCCTCTCAGGCGAAATCATGGGTA6030.16365412799218368No Hit
TGCCCATGTGCAGTAGATTACTGTAC6000.16283992835043154No Hit
CCACTACTCAGCAACTCAGTCAAGCA5980.16229712858926343No Hit
GATCGCGAGGCTAGGTCTGGCGAGTG5870.15931172990283884No Hit
GAGCAGACATGAGCGACATACCTCGT5790.15714053085816643No Hit
GTCACAAAGAATTCCCCCGACTGCAG5750.1560549313358302No Hit
TCTATTGAGATGTGTATGATGCGATT5660.15361233241057373No Hit
CGTCACTTCCACGAATGTGAGCCTTT5610.15225533300765348No Hit
TATGCCCTCTGCTTGCAACTACTGTG5600.15198393312706943No Hit
TTTCCTCTCAGGCGAACTAGTATCTT5590.15171253324648537No Hit
TAGTTGGTCACGACTATACGCGCCAG5470.14845573467947676No Hit
CCACTACTCAGCAACTTTAAGCCTTC5350.1451989361124681No Hit
CAGTAACTCCGCAAGCACTTGTCACT5300.14384193670954784No Hit
GAGGTGATCTCGTTTACATAAATCTA5300.14384193670954784No Hit
GCAATCAAGGACAGCTGCATTCCTTA5300.14384193670954784No Hit
TCTATTGAGATGTGTATGGTCCTGTG5140.139499538620203No Hit
TATGCCCTCTGCTTGCGTGGGGGCCT5090.13814253921728273No Hit
CAAGAAATCGTTTAGGGCATTGTACC5050.13705693969494653No Hit
GATCGCGAGGCTAGGTCTAGCAGTCC5020.13624274005319437No Hit
GTGGGTCGTCTAGGTTACGTCGGCCG5010.1359713401726103No Hit
TATGCCCTCTGCTTGCACTTCTGTAA4970.13488574065027412No Hit
GAGGTGATCTCGTTTATCCTACCTGG4910.1332573413667698No Hit
GCCTCTACATGGATGGGCGGTACGGG4910.1332573413667698No Hit
CCACTACTCAGCAACTTCGAGGATTC4770.12945774303859306No Hit
TGACTAGGTAGAGCTGGGGTGTGCGG4680.1270151441133366No Hit
CCACTACTCAGCAACTCTAGGTCCCT4570.12402974542691202No Hit
TAGTTGGTCACGACTATGGAAGGTCA4560.12375834554632795No Hit
GATCGCGAGGCTAGGTCCCCCAGTCT4520.12267274602399175No Hit
GCCTCTACATGGATGGCTTAACTACT4510.12240134614340771No Hit
GATCGCGAGGCTAGGTGCCGACGGCT4450.12077294685990338No Hit
GACGGCTTCGCCCTTATATACACCGT4420.11995874721815122No Hit
TATGCCCTCTGCTTGCGTGTCGTCTC4410.11968734733756718No Hit
GACGGCTTCGCCCTTAGGTCTGGAGA4350.11805894805406285No Hit
AGCAGCCCATCCAACACTCGTATGCT4340.1177875481734788No Hit
TCTATTGAGATGTGTAGCGGTGATGG4290.11643054877055854No Hit
GACACGCTCGTACCGGATACTTGACT4250.11534494924822233No Hit
TCTATTGAGATGTGTAAAGGTCTAAT4190.113716549964718No Hit
TGCCCATGTGCAGTAGTCGTGTTCTC4160.11290235032296585No Hit
GCCTCTACATGGATGGGCCAGGTGCC3980.1080171524724529No Hit
GACGGCTTCGCCCTTACCGTAACCGA3930.10666015306953266No Hit
GTCTCGTTCTTGCCGTCGGTAAACGC3810.10340335450252401No Hit
TCTATTGAGATGTGTAGGTCATACAG3810.10340335450252401No Hit
TCTCATAGTTGGTTTGTACACATTCT3800.10313195462193997No Hit
GCCTCTACATGGATGGTCCAACATTT3770.10231775498018782No Hit
CGGAGTCGTGGTACAGCGGGGCAGGA3720.10096075557726755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)