FastQCFastQC Report
Thu 14 Mar 2024
HYFNCDRX3_n01_CITE-seq_test_BCells_MixFACS_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFNCDRX3_n01_CITE-seq_test_BCells_MixFACS_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACTTGTCCATGATAATCGGGGCG591.0801904064445258No Hit
TTAACTCAGTGGTCCCCCGGGCAGAG400.7323324789454413No Hit
ACACCAAATGTCTGAACCTGCGGTTC360.6590992310508972No Hit
ACGATACTCATGTGGTTTTACTTCTT330.6041742951299891No Hit
ACGGGCTTCAGAGGTGACCATCGTGA300.5492493592090809No Hit
ATAGACCTCTGTCAAGGAGTTACTCT280.5126327352618089No Hit
ACGCCAGAGTAACCCTACGTGCATAC250.4577077993409008No Hit
CAGGTGCAGAACAATCCCACGCGGCC230.4210911753936287No Hit
CAAGATCGTCTAGGTTTTAGTCTAGG200.36616623947272064No Hit
GCCTCTAGATAGTCAAGTTCGACCGT140.25631636763090443No Hit
ACACCAACTAAGAACGGCCATGCACC120.21969974368363238No Hit
AGTAGCGACGGGCGGTGTGTACAAAG110.20139143170999635No Hit
TTTCCTCTAGATAGTCAAGTTCGACC100.18308311973636032No Hit
TCTATGGCCACCGTCCTGCTGTCTAT90.1647748077627243No Hit
ACACGACGCTCTTCCGATCTACACGA80.14646649578908824No Hit
GCCTCAGTTCCGAAAACCAACAAAAT70.12815818381545221No Hit
TATGCCCTTCTGCTCCACGGGAGGTT70.12815818381545221No Hit
ACACCAAGATCTGCACCTGCGGCGGC70.12815818381545221No Hit
GAGGTGATGTTTTTGGTAAACAGGCG60.10984987184181619No Hit
CAGTCAATGAAGCCTATGGCCTCCTG60.10984987184181619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)