FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_sp3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_sp3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50501433
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG5624491.1137287926067365TruSeq Adapter, Index 14 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2340680.46348783805798144TruSeq Adapter, Index 14 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGA1199010.23742098565797135TruSeq Adapter, Index 14 (98% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTT1083770.2146018311995226TruSeq Adapter, Index 14 (98% over 62bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG807820.1599598173778554RNA PCR Primer, Index 14 (97% over 43bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGA764150.1513125380026345TruSeq Adapter, Index 14 (98% over 63bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG762220.1509303706292849No Hit
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTG699460.13850300049901554TruSeq Adapter, Index 14 (98% over 63bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTG570620.11299085315064228TruSeq Adapter, Index 14 (98% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG563740.1116285155710334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA559800.053.7063960
TCGGGGG138000.051.7836654
CGTGAAA611700.049.17444261
ATCGGGG138700.046.972683
AAAAATG1895100.044.56539569
GAAAAAA3332750.043.5216264
TGAAAAA3351050.042.9117663
ACGTGTG495750.042.887581
CGGGGGG178900.042.3738755
GTGCGTG177750.041.84559258
CTTGAAA2640450.041.5289261
GATCGGG176400.040.1598172
CGTGTGC1264150.040.072631
GTGAAAA811600.039.5215462
GCCGTGT353900.038.68853460
TGCGTGG164500.038.5602769
TTGAAAA2902250.038.4852862
GTGTGCT1320300.038.199152
TGCCGTG902850.036.86396869
CGTGTTC340250.034.9523462
Produced by FastQC (version 0.11.2)