FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_sp2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_sp2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24133324
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG3269791.3548858831050377TruSeq Adapter, Index 13 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1113780.4615112282087623TruSeq Adapter, Index 13 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGA662880.27467413937673896TruSeq Adapter, Index 13 (98% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTT376120.15585088900310623TruSeq Adapter, Index 13 (98% over 62bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGG269430.11164230836995352TruSeq Adapter, Index 13 (98% over 63bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTG260080.10776799747933605TruSeq Adapter, Index 13 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG45400.046.793584
ACGTGTG210150.040.9734571
GAAAAAA1359150.040.67314564
ATCGGGG47600.039.994793
CGGGGGG56150.039.3087235
TGAAAAA1432950.038.41074863
CTTGAAA1356500.038.11328561
AAAAATG778950.037.6693469
TTGAAAA1437650.036.13953862
GCGTGAA84800.035.6869260
GATCGGG60450.032.405712
CGTGTGC370700.031.9290141
TGCTTGA3483050.030.11749869
GTGTGCT394400.029.8185792
AAAAAAT1143050.028.14304268
CTGCTTG3774100.027.99591368
ACACTCT146700.027.9130971
TGCCGTG93450.026.96000769
TGCGTGA109100.026.76088359
GCTTGAA1986450.025.98970669
Produced by FastQC (version 0.11.2)