FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_sp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_sp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19111573
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG46000.023.9171755
AGGGGGG29050.021.1325025
CGGGGGG15050.019.9369985
TCGGGGG12200.019.223224
TAGGGGG18050.017.3876654
TTGGGGG43550.017.343354
GAGCGGT24850.08.18791110
ATGGGGG33350.07.85949754
TTTGGGG65600.07.78105263
TTAGGGG28650.07.2228243
CACGTGA31950.06.8000937
AAGGGGG41800.06.6007114
AGAGCGG31200.06.4108619
ATCGGGG21250.06.3297443
TCACGTG36000.06.3228986
GCGACTC38100.06.15804561
CCTAGCG38700.06.154384657
CCCCGGA32000.06.14524841
GCGGTTC34600.05.98037412
GGTCTCG40950.05.732193555
Produced by FastQC (version 0.11.2)