FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21334025
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG848310.3976324205113662TruSeq Adapter, Index 22 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGA300450.14083137148287772TruSeq Adapter, Index 22 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG283380.1328300684001261TruSeq Adapter, Index 22 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1650550.037.95306469
TCGGGGG26900.035.6414074
CTGCTTG1852650.033.93412468
TCTGCTT2165150.029.25299367
GCTTGAA762350.029.01873869
CTTCTGC2215900.028.87677865
TTCTGCT2202250.028.73846666
CGTCTTC2303500.027.869462
GTCTTCT2308050.027.8117363
TCTTCTG2315050.027.68519864
CGGGGGG39250.027.150585
GCCGTCT2375750.027.0615960
CCGTCTT2383900.026.92758861
TGCCGTC2450300.026.31326959
CGTGTGC174700.026.145681
ATGCCGT2480850.026.1049358
GAGATCG699300.025.7857510
TATGCCG2540650.025.53168557
GTATGCC2571950.025.2916556
ATCGGGG30650.025.205313
Produced by FastQC (version 0.11.2)