FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23929585
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG1597880.6677424618939275TruSeq Adapter, Index 21 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG465000.19432012715640493TruSeq Adapter, Index 21 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGA307650.12856470348315693TruSeq Adapter, Index 21 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA730350.034.72495361
ACGTGTG155700.032.1926081
TGCTTGA1586000.032.14431469
TGAAAAA839200.031.069863
GAAAAAA854800.030.755864
TTGAAAA837850.030.51052362
CGTGTGC252550.030.3648571
CTGCTTG1738600.029.38442468
GCTTGAA874500.029.03080260
TCGGGGG30350.028.8631924
CGGGGGG35800.028.033575
GTGTGCT281850.027.1039892
AAAAATG533500.026.80731669
TCTGCTT2006250.025.66895367
CTTCTGC2033650.025.53606865
CGTCTTC2078150.025.03193362
TTCTGCT2065850.024.96254566
TCTTCTG2091450.024.81383564
GTCTTCT2123150.024.57928563
TGTGCTC436800.024.3483811
Produced by FastQC (version 0.11.2)