FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22625795
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG248070.10964034633921149TruSeq Adapter, Index 7 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA101850.040.24641860
TCGGGGG38600.037.9756664
GCCGTGT89050.033.1286760
CGGGGGG57600.031.1375685
CTGCGTG188900.029.9798968
CGTGAAA139450.029.49143661
TGCGTGG72550.029.444869
GCCGGGT48900.028.43596360
TGCCGGG58850.027.8531159
ATGCCGG129150.027.15265358
GATCGGG44800.027.0239142
TTCTGCG222200.026.79004966
CCCTACA70500.026.70951710
CTACACG70400.026.5522112
TCCCTAC71400.026.3243669
TGCGTGA142950.026.07150759
ACACGAC75350.025.95252414
CCTACAC72200.025.9376111
CTGCTTG657600.025.80953268
GCCGGCT100100.025.12842460
Produced by FastQC (version 0.11.2)