FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATG330.20482899882068153TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAA270.1675873626714667TruSeq Adapter, Index 3 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.16138042331326422No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGCAT220.13655266588045434TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCAGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA210.13034572652225188TruSeq Adapter, Index 3 (97% over 40bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210.13034572652225188No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA200.1241387871640494TruSeq Adapter, Index 3 (100% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190.11793184780584692No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC190.11793184780584692TruSeq Adapter, Index 14 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG180.11172490844764446TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAT170.10551796908944198TruSeq Adapter, Index 3 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC306.101901E-446.03738468
ACTTAGG404.7612724E-543.16004667
GTCACTT503.6690926E-641.3049764
TTAGGCA350.001303317639.46061369
CACTTAG501.7726545E-434.52803866
GCTTGAA601.2551802E-534.52803861
AGTCACT601.2824667E-534.4208163
CGTGTGC400.002549933534.4208071
TCACTTA501.8056159E-434.42080765
CTTGAAA652.2181537E-531.77305462
TGCTTGA703.5257737E-529.68794660
AAAAATG500.007483804627.62242969
TTGAAAA755.8918467E-527.53664463
GGTCAAA700.001271418324.66288436
TCACAGG700.001271418324.66288431
TCTTCCG1151.5622572E-623.9449122
CTTCCGA1151.5622572E-623.9449123
CTGCTTG1052.091854E-523.01869269
AGGTCAA750.001900180123.0186935
CTCTTCC1202.262539E-622.9472061
Produced by FastQC (version 0.11.2)