FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20622724
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG304600.14770114753026808TruSeq Adapter, Index 19 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG22650.033.1957324
CGGGGGG24200.032.209735
GCGTGAA61900.026.7573260
CTGCTTG524050.026.27678568
TGCTTGA493700.025.8369369
CTGCGTG82150.025.20668468
GCCGTCT651050.023.91899960
TATGCCG710900.023.80693257
GTATGCC748000.023.53586856
ATGCCGT696400.023.48819258
CGTATGC752700.023.35221755
GAGATCG202550.023.1389310
TGCCGTC678700.023.13501559
TCGTATG762550.022.93762654
CCGTCTT690000.022.74205261
CTCGTAT759550.022.67378853
TGGGGGG70250.022.6333355
CAGATCG326100.022.63179810
CTTCTGC706700.022.37833865
TCTCGTA779700.022.3308952
Produced by FastQC (version 0.11.2)