FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21032599
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG299400.022.26340957
GCCGTCT275100.022.02622660
CGGGGGG22550.021.8707855
GTATGCC319200.021.75883356
TCGGGGG20150.021.7371964
CATCTCG326300.021.46185550
CGTATGC323350.021.40494355
ATGCCGT300650.021.1474358
CTGCTTG257450.020.97923568
TGCCGTC296700.020.66919759
TCGTATG334050.020.48173954
GCATCTC350150.020.38356249
CGCATCT351050.020.3601248
CTCGTAT330800.020.33854753
TGGGGGG66450.020.189685
CGTCCGC377300.020.11078844
CCGTCTT304650.020.0463861
ACGTCCG380350.019.97664343
GTCCGCA386250.019.67234245
GAGATCG113500.019.50629410
Produced by FastQC (version 0.11.2)