FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11381
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCT208.156935E-569.032
AGCTCCA208.156935E-569.035
GTCGCTT208.156935E-569.042
CCAGTCG208.156935E-569.039
GGCGTCT150.002295770269.050
AGGCGTC150.002295770269.049
GAGCTCC208.156935E-569.034
CCGTCGT150.002295770269.063
TCGCTTA208.156935E-569.043
GTCTCGT150.002295770269.053
GCGTCTC150.002295770269.051
ATATGCC150.002295770269.058
TTAGGCG150.002295770269.047
CAGGTCT150.002295770269.027
ACAGGTC150.002295770269.026
GCTCCAG208.156935E-569.036
TCTCATA150.002295770269.054
GCACAGG150.002295770269.024
TGAGCTC208.156935E-569.033
CATATGC150.002295770269.057
Produced by FastQC (version 0.11.2)