FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_ry1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_ry1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17769698
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG20150.027.3845255
CTGCTTG324650.026.01374468
TATGCCG429350.025.47940357
GAGATCG133850.025.37735710
TCGGGGG20300.025.3134024
GTATGCC449550.025.06457756
TGCTTGA302450.025.00197469
CGTATGC453950.024.87514955
CATCTCG408550.024.81253450
ATGCCGT420600.024.66977358
TCGTATG451500.024.52112854
GCCGTCT386100.024.47967160
CTGCGTG67350.024.44359468
CTCGTAT414050.023.7704653
TGCCGTC409700.023.61983159
CCGTCTT409350.023.18796761
ACCCGTC543600.023.17553943
TCACCCG548250.023.140441
CGTCCAT529100.023.11479846
ATCTCGT436900.023.03016751
Produced by FastQC (version 0.11.2)