FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23967092
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG2945721.2290685912166566RNA PCR Primer, Index 24 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1348910.5628175499973046RNA PCR Primer, Index 24 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGA224650.056.9156359
GCGTGAA239000.056.29405260
CTGCGTG195150.054.3712658
GTGCGTG59300.052.8524758
CGTGAAA265450.051.14743461
GGCATTC2853450.047.8592722
GCATTCC2897450.047.6063583
CGGCATT2828800.047.4434851
CATTCCT2959400.046.7111854
ATTCCTG2968250.046.5927355
CTTGAAA1158350.046.30636661
CTCGGCA772800.046.130541
GCTTGAA1163800.045.94014760
TCCTGCT3015900.045.7628067
TTCCTGC3025700.045.6465076
TGCTTGA1147200.045.53854459
GAACCGC3015500.045.49630414
CCTGCTG3039150.045.4569668
ACCGCTC3013600.045.2469916
TGAACCG3038950.045.19813
Produced by FastQC (version 0.11.2)