FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21203873
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG2128951.0040382716874412RNA PCR Primer, Index 23 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1081900.5102369741603339RNA PCR Primer, Index 23 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGA293900.056.7952759
GCGTGAA312600.056.6149660
GTGCGTG85100.055.93215658
CGTGAAA333000.053.4006761
CTGCGTG258300.052.5698658
CTTGAAA916050.050.4020761
GCTTGAA944650.048.6907860
TTCTGCT4102650.048.57179668
TCTGCTT4175800.048.54030669
TGTGCGT105700.048.30086557
GTCTTCT4343550.047.02530765
CTTCTGC4382150.046.7997267
CGTCTTC4476100.046.61147764
TCTTCTG4420450.046.5348166
TGCTTGA969350.046.2093459
GCCGTCT4664650.044.6334762
CCGTCTT4754950.044.607963
TGCCGTC4833250.043.82827861
AAAAATG865050.043.57808369
TTGAAAA1086200.043.48844562
Produced by FastQC (version 0.11.2)