FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21126757
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG4198641.9873566018674804TruSeq Adapter, Index 3 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2017810.9550968944263428TruSeq Adapter, Index 3 (100% over 63bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG317830.15043955870747222TruSeq Adapter, Index 3 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308130.1458482245997339No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG267310.1265267546741793TruSeq Adapter, Index 3 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAG231420.10953881847554738TruSeq Adapter, Index 3 (98% over 63bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222780.10544921778576806No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATT215010.1017714171654457TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211900.1002993502504904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA492700.061.9114360
TGCGTGA465400.061.3961459
GTGCGTG138550.060.4628858
CGTGAAA517850.059.3322761
CTGCGTG390450.058.38042458
CTTGAAA1627050.056.45479661
TGTGCGT163700.055.56387757
GCTTGAA1646150.055.52192760
AAAAATG1360600.053.85745269
TGCTTGA1673500.053.15289759
TTGAAAA1803000.051.8898362
TGAAAAA2486100.051.26293663
TTGTGCG151950.051.02403656
GTGAAAA650050.050.8245762
GAAAAAA2553250.050.2711164
CTTGTGC295600.047.57538655
TCTTGTG299300.047.55261254
GTCTTGT290600.047.14758353
TCTGCGT523950.045.67640757
TCGGGGG48600.045.6294824
Produced by FastQC (version 0.11.2)