FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24090620
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG2357920.9787709905349053TruSeq Adapter, Index 2 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1368860.5682128562901245TruSeq Adapter, Index 2 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA422250.059.52386560
GTGCGTG128900.059.2829958
TGCGTGA404750.058.95620759
CGTGAAA448650.056.32413561
CTGCGTG343350.054.11189358
TGTGCGT151900.052.51394757
CTTGAAA1171850.051.2160761
GCTTGAA1191700.050.09218660
TTCTGCT5331450.049.1893568
TCTGCTT5435450.049.10724669
TTGTGCG143450.048.1492856
TGCTTGA1213900.047.9546459
AAAAATG1064350.047.73946469
GTCTTCT5662600.047.47924465
CTTCTGC5733200.047.3586167
TCTTCTG5764800.047.1260966
CGTCTTC5824350.047.11586864
GTGAAAA587600.046.1318562
CCGTCTT6203400.045.02746263
TTGAAAA1364850.045.0047762
Produced by FastQC (version 0.11.2)