FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19861016
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT571450.042.98307865
TCTGCTT589550.041.24761669
CTCGTAT562300.039.83846755
CCGTCTT645350.039.51352763
CGTCTTC640600.039.36865264
GCCGTCT638000.038.8856262
TCTCGTA578850.038.88431554
TCTTCTG643250.038.6763866
TTCTGCT619100.038.65419468
CTTGAAA136650.037.47097861
TCGTATG681550.036.98081256
CTTCTGC670250.036.9709167
ATCTCGT612800.036.8313853
GCTTGAA141000.036.1461260
GTATGCC713650.036.0484258
CGTATGC719700.035.5518857
ATGCCGT727500.034.7815560
TGCCGTC727800.034.7553761
TATGCCG733850.034.72380459
GATCTCG657600.034.50522652
Produced by FastQC (version 0.11.2)