FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_mk1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_mk1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21339622
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG736120.3449545638624714TruSeq Adapter, Index 4 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG266930.1250865643262097TruSeq Adapter, Index 4 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA247900.049.5208761
GCTTGAA263600.046.52298460
TGCTTGA299400.040.8604159
GTCTTCT805000.036.70494565
TCTGCTT815700.035.9447669
TTGAAAA348300.035.6497762
CCGTCTT868900.034.25162563
TCTTCTG868400.034.0476266
CGTCTTC876600.033.95708564
CTGCTTG355050.033.87831558
TTCTGCT866350.033.8513468
GCCGTCT881000.033.7449362
CTTCTGC890850.033.18758867
GTATGCC944700.031.70547558
CTCGTAT933400.031.5487755
TCGTATG950500.031.50392256
CGTATGC958800.031.25919557
TCTCGTA949850.030.99556254
TGCCGTC970500.030.80931361
ATGCCGT973800.030.7500260
Produced by FastQC (version 0.11.2)