FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_jo4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_jo4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17749311
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG20450.034.909764
CGGGGGG25100.030.9157545
CCGGCGT30350.027.86531857
TATCGCG74550.027.77380246
ATCGCGT66900.027.13328647
TCGCGTA55200.027.1332648
GAGCGGT109250.024.59002110
TGGGGGG48950.024.4482525
CTGCTTG137250.024.15996264
AGAGCGG113650.023.7893969
TGCCGGC36500.023.35974355
CCGAGAG48200.023.19015329
GCGGTTC118850.023.15561912
TCCGGCG20300.023.12633756
GATCGGG23200.022.8935742
TGCCGAG68250.022.49032427
ATGCCGA69650.022.4827726
GCTTGAA156900.022.03939866
CGGCGTC31400.021.98530458
GCTTATC266100.021.35735143
Produced by FastQC (version 0.11.2)