FastQCFastQC Report
Tue 14 Mar 2017
HYFH2BGXY_n01_jo3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYFH2BGXY_n01_jo3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18804772
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG50100.027.8098185
TCGGGGG16700.027.6721464
CGGGGGG18850.026.8943165
AGGGGGG27650.019.582095
TTGGGGG48900.019.394494
TAGGGGG18750.014.3465624
GAGCGGT34950.012.53139710
AGAGCGG39600.011.40794859
CCCTACG25600.011.0510940
TTCGGGG25600.010.777483
ATCGGGG21000.010.346383
GCGGTTC48000.010.2024112
ATGGGGG35900.09.8945784
TACGGCC28500.09.68566643
CTACGGC29550.09.57471242
TTTGGGG71950.08.9155753
AAGAGCG53100.08.7673748
ACGGCCT33600.08.421020544
AGATCGG120300.08.2583961
AGCGGTT59700.08.02955611
Produced by FastQC (version 0.11.2)